Composite
+Rhl senso

Part:BBa_K1157018

Designed by: Lin Ang Chieh   Group: iGEM13_NTU-Taida   (2013-09-15)

pRhl-RhlR-mCherry-Pc-RhlR

This is a biosensor that senses C-4 AHL molecules. This design contains a positive feedback and expresses mCherry reporter when C-4 AHL binds with Rhl receptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1706
    Illegal NheI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 322
    Illegal BamHI site found at 2003
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 797
    Illegal BsaI site found at 2478


[edit]
Categories
Parameters
None